A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves

We herein developed a protocol for the rapid procurement of adult nerve-derived Schwann cells (SCs) that was optimized to implement an immediate enzymatic dissociation of fresh nerve tissue while maintaining high cell viability, improving yields and minimizing fibroblast and myelin contamination. Th...

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Detalles Bibliográficos
Autores principales: Andersen, Natalia D., Srinivas, Shruthi, Piñero, Gonzalo, Monje, Paula V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994039/
https://www.ncbi.nlm.nih.gov/pubmed/27549422
http://dx.doi.org/10.1038/srep31781

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994039/
https://www.ncbi.nlm.nih.gov/pubmed/27549422
http://dx.doi.org/10.1038/srep31781

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