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Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix were sequenced. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts with known concentration. cDNA librar...

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Detalles Bibliográficos
Autores principales: Oikonomopoulos, Spyros, Wang, Yu Chang, Djambazian, Haig, Badescu, Dunarel, Ragoussis, Jiannis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995519/
https://www.ncbi.nlm.nih.gov/pubmed/27554526
http://dx.doi.org/10.1038/srep31602