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A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism
The clustered regularly interspersed palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9) system is a powerful tool for genome editing that can be used to construct a guide RNA (gRNA) library for genetic screening. For gRNA design, one must know the sequence of the 20-mer flanking the pro...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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American Association for the Advancement of Science
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996643/ https://www.ncbi.nlm.nih.gov/pubmed/27574704 http://dx.doi.org/10.1126/sciadv.1600699 |