Cargando…
Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
The Hippo pathway is dysregulated in multiple types of human cancer, including ovarian cancer. Nuclear expression of yes-associated protein 1 (YAP1), a downstream transcription coactivator of the Hippo pathway, has been demonstrated to promote tumorigenesis in ovarian cancer and may serve as a poor...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997911/ https://www.ncbi.nlm.nih.gov/pubmed/27588129 http://dx.doi.org/10.3892/ol.2016.4829 |
_version_ | 1782449853103603712 |
---|---|
author | Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong |
author_facet | Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong |
author_sort | Chen, Guangyuan |
collection | PubMed |
description | The Hippo pathway is dysregulated in multiple types of human cancer, including ovarian cancer. Nuclear expression of yes-associated protein 1 (YAP1), a downstream transcription coactivator of the Hippo pathway, has been demonstrated to promote tumorigenesis in ovarian cancer and may serve as a poor prognostic indicator. However, transcriptional coactivator with PDZ binding motif (TAZ), a downstream target of the Hippo pathway and paralog of YAP in mammalian cells, has not been fully investigated in ovarian cancer. The present study aimed to investigate the dysregulation and biological function of TAZ in ovarian cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting revealed that TAZ mRNA and protein levels, respectively, were upregulated in ovarian cancer, and a meta-analysis of ovarian cancer microarray datasets identified that increased expression of TAZ mRNA is correlated with poor prognosis in patients with ovarian cancer. In addition, TAZ-knockdown in ovarian cancer cells demonstrated that TAZ regulates the migration, proliferation and epithelial-mesenchymal transition of ovarian cancer cells. Furthermore, pharmacological disruption of the YAP/TAZ/TEA domain protein complex resulted in a decrease in ovarian cancer cell migration, proliferation and vimentin expression. The results of the present study indicate that the overexpression of TAZ is important in the development and progression of ovarian cancer, and may function as a potential drug target for treatment of this disease entity. |
format | Online Article Text |
id | pubmed-4997911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-49979112016-09-01 Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong Oncol Lett Articles The Hippo pathway is dysregulated in multiple types of human cancer, including ovarian cancer. Nuclear expression of yes-associated protein 1 (YAP1), a downstream transcription coactivator of the Hippo pathway, has been demonstrated to promote tumorigenesis in ovarian cancer and may serve as a poor prognostic indicator. However, transcriptional coactivator with PDZ binding motif (TAZ), a downstream target of the Hippo pathway and paralog of YAP in mammalian cells, has not been fully investigated in ovarian cancer. The present study aimed to investigate the dysregulation and biological function of TAZ in ovarian cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting revealed that TAZ mRNA and protein levels, respectively, were upregulated in ovarian cancer, and a meta-analysis of ovarian cancer microarray datasets identified that increased expression of TAZ mRNA is correlated with poor prognosis in patients with ovarian cancer. In addition, TAZ-knockdown in ovarian cancer cells demonstrated that TAZ regulates the migration, proliferation and epithelial-mesenchymal transition of ovarian cancer cells. Furthermore, pharmacological disruption of the YAP/TAZ/TEA domain protein complex resulted in a decrease in ovarian cancer cell migration, proliferation and vimentin expression. The results of the present study indicate that the overexpression of TAZ is important in the development and progression of ovarian cancer, and may function as a potential drug target for treatment of this disease entity. D.A. Spandidos 2016-09 2016-07-08 /pmc/articles/PMC4997911/ /pubmed/27588129 http://dx.doi.org/10.3892/ol.2016.4829 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer |
title | Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer |
title_full | Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer |
title_fullStr | Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer |
title_full_unstemmed | Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer |
title_short | Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer |
title_sort | overexpression of taz promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997911/ https://www.ncbi.nlm.nih.gov/pubmed/27588129 http://dx.doi.org/10.3892/ol.2016.4829 |
work_keys_str_mv | AT chenguangyuan overexpressionoftazpromotescellproliferationmigrationandepithelialmesenchymaltransitioninovariancancer AT xiejiabin overexpressionoftazpromotescellproliferationmigrationandepithelialmesenchymaltransitioninovariancancer AT huangping overexpressionoftazpromotescellproliferationmigrationandepithelialmesenchymaltransitioninovariancancer AT yangzhihong overexpressionoftazpromotescellproliferationmigrationandepithelialmesenchymaltransitioninovariancancer |