CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA

Current DNA assembly methods for preparing highly purified linear subassemblies require complex and time-consuming in vitro manipulations that hinder their ability to construct megabase-sized DNAs (e.g. synthetic genomes). We have developed a new method designated ‘CasHRA (Cas9-facilitated Homologou...

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Detalles Bibliográficos
Autores principales: Zhou, Jianting, Wu, Ronghai, Xue, Xiaoli, Qin, Zhongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001600/
https://www.ncbi.nlm.nih.gov/pubmed/27220470
http://dx.doi.org/10.1093/nar/gkw475

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001600/
https://www.ncbi.nlm.nih.gov/pubmed/27220470
http://dx.doi.org/10.1093/nar/gkw475

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