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CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA
Current DNA assembly methods for preparing highly purified linear subassemblies require complex and time-consuming in vitro manipulations that hinder their ability to construct megabase-sized DNAs (e.g. synthetic genomes). We have developed a new method designated ‘CasHRA (Cas9-facilitated Homologou...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001600/ https://www.ncbi.nlm.nih.gov/pubmed/27220470 http://dx.doi.org/10.1093/nar/gkw475 |