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Induction of Silencing in Plants by High-Pressure Spraying of In vitro-Synthesized Small RNAs

In this report, we describe a method for the delivery of small interfering RNAs (siRNAs) into plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a GREEN FLUORESCENT PROTEIN (GFP) transgene were applied by various methods onto GFP-expressing transgenic Nicotian...

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Detalles Bibliográficos
Autores principales: Dalakouras, Athanasios, Wassenegger, Michèle, McMillan, John N., Cardoza, Vinitha, Maegele, Ira, Dadami, Elena, Runne, Miriam, Krczal, Gabi, Wassenegger, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003833/
https://www.ncbi.nlm.nih.gov/pubmed/27625678
http://dx.doi.org/10.3389/fpls.2016.01327