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AβPP processing results in greater toxicity per amount of Aβ(1-42) than individually expressed and secreted Aβ(1-42) in Drosophila melanogaster
The aggregation of the amyloid-β (Aβ) peptide into fibrillar deposits has long been considered the key neuropathological hallmark of Alzheimer's disease (AD). Aβ peptides are generated from proteolytic processing of the transmembrane Aβ precursor protein (AβPP) via sequential proteolysis throug...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004604/ https://www.ncbi.nlm.nih.gov/pubmed/27387531 http://dx.doi.org/10.1242/bio.017194 |
Sumario: | The aggregation of the amyloid-β (Aβ) peptide into fibrillar deposits has long been considered the key neuropathological hallmark of Alzheimer's disease (AD). Aβ peptides are generated from proteolytic processing of the transmembrane Aβ precursor protein (AβPP) via sequential proteolysis through the β-secretase activity of β-site AβPP-cleaving enzyme (BACE1) and by the intramembranous enzyme γ-secretase. For over a decade, Drosophila melanogaster has been used as a model organism to study AD, and two different approaches have been developed to investigate the toxicity caused by AD-associated gene products in vivo. In one model, the Aβ peptide is directly over-expressed fused to a signal peptide, allowing secretion of the peptide into the extracellular space. In the other model, human AβPP is co-expressed with human BACE1, resulting in production of the Aβ peptide through the processing of AβPP by BACE1 and by endogenous fly γ-secretase. Here, we performed a parallel study of flies that expressed the Aβ(1-42) peptide alone or that co-expressed AβPP and BACE1. Toxic effects (assessed by eye phenotype, longevity and locomotor assays) and levels of the Aβ(1-42), Aβ(1-40) and Aβ(1-38) peptides were examined. Our data reveal that the toxic effect per amount of detected Aβ(1-42) peptide was higher in the flies co-expressing AβPP and BACE1 than in the Aβ(1-42)-expressing flies, and that the co-existence of Aβ(1-42) and Aβ(1-40) in the flies co-expressing AβPP and BACE1 could be of significant importance to the neurotoxic effect detected in these flies. Thus, the toxicity detected in these two fly models seems to have different modes of action and is highly dependent on how and where the peptide is generated rather than on the actual level of the Aβ(1-42) peptide in the flies. This is important knowledge that needs to be taken into consideration when using Drosophila models to investigate disease mechanisms or therapeutic strategies in AD research. |
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