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New tools to convert bacterial artificial chromosomes to a self-excising design and their application to a herpes simplex virus type 1 infectious clone

BACKGROUND: Infectious clones are fundamental tools for the study of many viruses, allowing for efficient mutagenesis and reproducible production of genetically-defined strains. For the large dsDNA genomes of the herpesviridae, bacterial artificial chromosomes have become the cloning vector of choic...

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Detalles Bibliográficos
Autores principales:	Richards, Alexsia L., Sollars, Patricia J., Smith, Gregory A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006514/ https://www.ncbi.nlm.nih.gov/pubmed/27580861 http://dx.doi.org/10.1186/s12896-016-0295-4

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