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Detection of protein–protein interactions at the septin collar in Saccharomyces cerevisiae using a tripartite split-GFP system
Various methods can provide a readout of the physical interaction between two biomolecules. A recently described tripartite split-GFP system has the potential to report by direct visualization via a fluorescence signal the intimate association of minimally tagged proteins expressed at their endogeno...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007091/ https://www.ncbi.nlm.nih.gov/pubmed/27385335 http://dx.doi.org/10.1091/mbc.E16-05-0337 |