Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line

CRISPR/Cas9 system has been used widely in animals and plants to direct mutagenesis. To date, no such method exists for fish somatic cell lines. We describe an efficient procedure for genome editing in the Chinook salmon Oncorhynchus tshawytscha CHSE. This cell line was genetically modified to first...

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Detalles Bibliográficos
Autores principales: Dehler, Carola E., Boudinot, Pierre, Martin, Samuel A. M., Collet, Bertrand
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007268/
https://www.ncbi.nlm.nih.gov/pubmed/27236514
http://dx.doi.org/10.1007/s10126-016-9708-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007268/
https://www.ncbi.nlm.nih.gov/pubmed/27236514
http://dx.doi.org/10.1007/s10126-016-9708-6

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