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Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line
CRISPR/Cas9 system has been used widely in animals and plants to direct mutagenesis. To date, no such method exists for fish somatic cell lines. We describe an efficient procedure for genome editing in the Chinook salmon Oncorhynchus tshawytscha CHSE. This cell line was genetically modified to first...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007268/ https://www.ncbi.nlm.nih.gov/pubmed/27236514 http://dx.doi.org/10.1007/s10126-016-9708-6 |