Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution

The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distri...

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Autores principales: Mudumbi, Krishna C, Schirmer, Eric C, Yang, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007294/
https://www.ncbi.nlm.nih.gov/pubmed/27558844
http://dx.doi.org/10.1038/ncomms12562
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author Mudumbi, Krishna C
Schirmer, Eric C
Yang, Weidong
author_facet Mudumbi, Krishna C
Schirmer, Eric C
Yang, Weidong
author_sort Mudumbi, Krishna C
collection PubMed
description The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs in vivo.
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spelling pubmed-50072942016-09-14 Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution Mudumbi, Krishna C Schirmer, Eric C Yang, Weidong Nat Commun Article The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs in vivo. Nature Publishing Group 2016-08-25 /pmc/articles/PMC5007294/ /pubmed/27558844 http://dx.doi.org/10.1038/ncomms12562 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mudumbi, Krishna C
Schirmer, Eric C
Yang, Weidong
Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
title Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
title_full Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
title_fullStr Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
title_full_unstemmed Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
title_short Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
title_sort single-point single-molecule frap distinguishes inner and outer nuclear membrane protein distribution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007294/
https://www.ncbi.nlm.nih.gov/pubmed/27558844
http://dx.doi.org/10.1038/ncomms12562
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AT yangweidong singlepointsinglemoleculefrapdistinguishesinnerandouternuclearmembraneproteindistribution

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