High-Throughput Screening of Coenzyme Preference Change of Thermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(+)

Coenzyme engineering that changes NAD(P) selectivity of redox enzymes is an important tool in metabolic engineering, synthetic biology, and biocatalysis. Here we developed a high throughput screening method to identify mutants of 6-phosphogluconate dehydrogenase (6PGDH) from a thermophilic bacterium...

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Detalles Bibliográficos
Autores principales: Huang, Rui, Chen, Hui, Zhong, Chao, Kim, Jae Eung, Zhang, Yi-Heng Percival
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009329/
https://www.ncbi.nlm.nih.gov/pubmed/27587230
http://dx.doi.org/10.1038/srep32644

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009329/
https://www.ncbi.nlm.nih.gov/pubmed/27587230
http://dx.doi.org/10.1038/srep32644

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