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A rapid method for quantifying free and bound acetate based on alkylation and GC-MS analysis
BACKGROUND: Acetyl-CoA is a key metabolic intermediate with roles in the production of energy and biomass, as well as in metabolic regulation. It was recently found that acetate is crucial for maintaining acetyl-CoA production in hypoxic cancer cells. However, the availability of free acetate in the...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009658/ https://www.ncbi.nlm.nih.gov/pubmed/27594997 http://dx.doi.org/10.1186/s40170-016-0157-5 |