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Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro
Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ~70% viability, can be routinely achieved even in primary cells like mesenchymal stem cells. H...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5014460/ https://www.ncbi.nlm.nih.gov/pubmed/27111417 http://dx.doi.org/10.1038/mtna.2016.4 |