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Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase

Middle East respiratory syndrome coronavirus (MERS-CoV) helicase is a superfamily 1 helicase containing seven conserved motifs. We have cloned, expressed, and purified a Strep-fused recombinant MERS-CoV nonstructural protein 13 (M-nsp13) helicase. Characterization of its biochemical properties showe...

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Detalles Bibliográficos
Autores principales: Adedeji, Adeyemi O., Lazarus, Hilary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5014916/
https://www.ncbi.nlm.nih.gov/pubmed/27631026
http://dx.doi.org/10.1128/mSphere.00235-16