Coma aberrations in combined two- and three-dimensional STED nanoscopy

Stimulated emission depletion (STED) microscopes, like all super-resolution methods, are sensitive to aberrations. Of particular importance are aberrations that affect the quality of the depletion focus, which requires a point of near-zero intensity surrounded by strong illumination. We present anal...

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Autores principales: Antonello, Jacopo, Kromann, Emil B., Burke, Daniel, Bewersdorf, Joerg, Booth, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017529/
https://www.ncbi.nlm.nih.gov/pubmed/27472636
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author Antonello, Jacopo
Kromann, Emil B.
Burke, Daniel
Bewersdorf, Joerg
Booth, Martin J.
author_facet Antonello, Jacopo
Kromann, Emil B.
Burke, Daniel
Bewersdorf, Joerg
Booth, Martin J.
author_sort Antonello, Jacopo
collection PubMed
description Stimulated emission depletion (STED) microscopes, like all super-resolution methods, are sensitive to aberrations. Of particular importance are aberrations that affect the quality of the depletion focus, which requires a point of near-zero intensity surrounded by strong illumination. We present analysis, modeling, and experimental measurements that show the effects of coma aberrations on depletion patterns of two-dimensional (2D) and three-dimensional (3D) STED configurations. Specifically, we find that identical coma aberrations create focal shifts in opposite directions in 2D and 3D STED. This phenomenon could affect the precision of microscopic measurements and has ramifications for the efficacy of combined 2D/3D STED systems.
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spelling pubmed-50175292016-09-09 Coma aberrations in combined two- and three-dimensional STED nanoscopy Antonello, Jacopo Kromann, Emil B. Burke, Daniel Bewersdorf, Joerg Booth, Martin J. Opt Lett Article Stimulated emission depletion (STED) microscopes, like all super-resolution methods, are sensitive to aberrations. Of particular importance are aberrations that affect the quality of the depletion focus, which requires a point of near-zero intensity surrounded by strong illumination. We present analysis, modeling, and experimental measurements that show the effects of coma aberrations on depletion patterns of two-dimensional (2D) and three-dimensional (3D) STED configurations. Specifically, we find that identical coma aberrations create focal shifts in opposite directions in 2D and 3D STED. This phenomenon could affect the precision of microscopic measurements and has ramifications for the efficacy of combined 2D/3D STED systems. 2016-08-01 /pmc/articles/PMC5017529/ /pubmed/27472636 Text en http://creativecommons.org/licenses/by/4.0/ Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
spellingShingle Article
Antonello, Jacopo
Kromann, Emil B.
Burke, Daniel
Bewersdorf, Joerg
Booth, Martin J.
Coma aberrations in combined two- and three-dimensional STED nanoscopy
title Coma aberrations in combined two- and three-dimensional STED nanoscopy
title_full Coma aberrations in combined two- and three-dimensional STED nanoscopy
title_fullStr Coma aberrations in combined two- and three-dimensional STED nanoscopy
title_full_unstemmed Coma aberrations in combined two- and three-dimensional STED nanoscopy
title_short Coma aberrations in combined two- and three-dimensional STED nanoscopy
title_sort coma aberrations in combined two- and three-dimensional sted nanoscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017529/
https://www.ncbi.nlm.nih.gov/pubmed/27472636
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