Application of Locked Nucleic Acid (LNA) Primer and PCR Clamping by LNA Oligonucleotide to Enhance the Amplification of Internal Transcribed Spacer (ITS) Regions in Investigating the Community Structures of Plant–Associated Fungi

The simultaneous extraction of host plant DNA severely limits investigations of the community structures of plant–associated fungi due to the similar homologies of sequences in primer–annealing positions between fungi and host plants. Although fungal-specific primers have been designed, plant DNA co...

Descripción completa

Detalles Bibliográficos
Autores principales: Ikenaga, Makoto, Tabuchi, Masakazu, Kawauchi, Tomohiro, Sakai, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017812/
https://www.ncbi.nlm.nih.gov/pubmed/27600711
http://dx.doi.org/10.1264/jsme2.ME16085

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017812/
https://www.ncbi.nlm.nih.gov/pubmed/27600711
http://dx.doi.org/10.1264/jsme2.ME16085

Ejemplares similares