Mechanistic insights from resolving ligand-dependent kinetics of conformational changes at ATP-gated P2X1R ion channels

Structural studies of P2X receptors show a novel U shaped ATP orientation following binding. We used voltage clamp fluorometry (VCF) and molecular dynamics (MD) simulations to investigate agonist action. For VCF the P2X1 receptor (P2X1R) K190C mutant (adjacent to the agonist binding pocket) was labe...

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Detalles Bibliográficos
Autores principales: Fryatt, Alistair G., Dayl, Sudad, Cullis, Paul M., Schmid, Ralf, Evans, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018734/
https://www.ncbi.nlm.nih.gov/pubmed/27616669
http://dx.doi.org/10.1038/srep32918

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018734/
https://www.ncbi.nlm.nih.gov/pubmed/27616669
http://dx.doi.org/10.1038/srep32918

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