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Serum proteins are extracted along with monolayer cells in plasticware and interfere with protein analysis
Washing and lysing monolayer cells directly from cell culture plasticware is a commonly used method for protein extraction. We found that multiple protein bands were enriched in samples with low cell numbers from the 6-well plate cultures. These proteins contributed to the overestimation of cell pro...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
jbm
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019348/ https://www.ncbi.nlm.nih.gov/pubmed/27631018 http://dx.doi.org/10.14440/jbm.2016.129 |