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Fluorescence imaging of chromosomal DNA using click chemistry

Chromosome visualization is essential for chromosome analysis and genetic diagnostics. Here, we developed a click chemistry approach for multicolor imaging of chromosomal DNA instead of the traditional dye method. We first demonstrated that the commercially available reagents allow for the multicolo...

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Detalles Bibliográficos
Autores principales: Ishizuka, Takumi, Liu, Hong Shan, Ito, Kenichiro, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020420/
https://www.ncbi.nlm.nih.gov/pubmed/27620982
http://dx.doi.org/10.1038/srep33217