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πSPIM: high NA high resolution isotropic light-sheet imaging in cell culture dishes

Light-sheet fluorescence microscopy (LSFM), also termed single plane illumination microscopy (SPIM), enables live cell fluorescence imaging with optical sectioning capabilities superior to confocal microscopy and without any out-of-focus exposure of the specimen. However, the need of two objective l...

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Detalles Bibliográficos
Autores principales: Theer, Patrick, Dragneva, Denitsa, Knop, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020645/
https://www.ncbi.nlm.nih.gov/pubmed/27619647
http://dx.doi.org/10.1038/srep32880