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Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells

Translation is an essential step in gene expression. In this study, we used an improved SunTag system to label nascent proteins and image translation of single messenger ribonucleoproteins (mRNPs) in human cells. Using a dedicated reporter RNA, we observe that translation of single mRNPs stochastica...

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Detalles Bibliográficos
Autores principales: Pichon, Xavier, Bastide, Amandine, Safieddine, Adham, Chouaib, Racha, Samacoits, Aubin, Basyuk, Eugenia, Peter, Marion, Mueller, Florian, Bertrand, Edouard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021097/
https://www.ncbi.nlm.nih.gov/pubmed/27597760
http://dx.doi.org/10.1083/jcb.201605024