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Development of a robust DNA quality and quantity assessment qPCR assay for targeted next-generation sequencing library preparation

Next-generation sequencing (NGS) is becoming a standard for genetic analyses of clinical samples. DNAs retrieved from formalin-fixed, paraffin-embedded (FFPE) tissue specimens are commonly degraded, and specimens such as core biopsies are sometimes too small to obtain enough DNA for NGS applications...

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Detalles Bibliográficos
Autores principales: Dang, Jennifer, Mendez, Pedro, Lee, Sharon, Kim, James W., Yoon, Jun-Hee, Kim, Thomas W., Sailey, Charles J., Jablons, David M., Kim, Il-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021246/
https://www.ncbi.nlm.nih.gov/pubmed/27511764
http://dx.doi.org/10.3892/ijo.2016.3654