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Rational engineering of a virulence gene from Mycobacterium tuberculosis facilitates proteomic analysis of a natural protein N-terminus

Mass spectrometry (MS) for the detection of proteins is an indispensable tool for evaluating the biological processes of the proteome. Proteomics frequently requires proteolysis of proteins into peptide fragments. Proteins can be refractory to ideal proteolysis at the sequence level rendering them d...

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Detalles Bibliográficos
Autores principales: Reyna, Cristal, Mba Medie, Felix, Champion, Matthew M., Champion, Patricia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021934/
https://www.ncbi.nlm.nih.gov/pubmed/27625110
http://dx.doi.org/10.1038/srep33265