Role of NAD(+)-Dependent Malate Dehydrogenase in the Metabolism of Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b

We have expressed the l-malate dehydrogenase (MDH) genes from aerobic methanotrophs Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b as his-tagged proteins in Escherichia coli. The substrate specificities, enzymatic kinetics and oligomeric states of the MDHs have been characteri...

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Detalles Bibliográficos
Autores principales: Rozova, Olga N., Khmelenina, Valentina N., Bocharova, Ksenia A., Mustakhimov, Ildar I., Trotsenko, Yuri A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023229/
https://www.ncbi.nlm.nih.gov/pubmed/27682078
http://dx.doi.org/10.3390/microorganisms3010047

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023229/
https://www.ncbi.nlm.nih.gov/pubmed/27682078
http://dx.doi.org/10.3390/microorganisms3010047

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