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Role of NAD(+)-Dependent Malate Dehydrogenase in the Metabolism of Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b

We have expressed the l-malate dehydrogenase (MDH) genes from aerobic methanotrophs Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b as his-tagged proteins in Escherichia coli. The substrate specificities, enzymatic kinetics and oligomeric states of the MDHs have been characteri...

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Detalles Bibliográficos
Autores principales:	Rozova, Olga N., Khmelenina, Valentina N., Bocharova, Ksenia A., Mustakhimov, Ildar I., Trotsenko, Yuri A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023229/ https://www.ncbi.nlm.nih.gov/pubmed/27682078 http://dx.doi.org/10.3390/microorganisms3010047

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