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Removing the bottlenecks of cell culture metabolomics: fast normalization procedure, correlation of metabolites to cell number, and impact of the cell harvesting method

INTRODUCTION: Although cultured cells are nowadays regularly analyzed by metabolomics technologies, some issues in study setup and data processing are still not resolved to complete satisfaction: a suitable harvesting method for adherent cells, a fast and robust method for data normalization, and th...

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Detalles Bibliográficos
Autores principales: Muschet, Caroline, Möller, Gabriele, Prehn, Cornelia, de Angelis, Martin Hrabě, Adamski, Jerzy, Tokarz, Janina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025493/
https://www.ncbi.nlm.nih.gov/pubmed/27729828
http://dx.doi.org/10.1007/s11306-016-1104-8