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TGF-β1 autocrine signalling and enamel matrix components
Transforming growth factor-β1 (TGF-β1) is present in porcine enamel extracts and is critical for proper mineralization of tooth enamel. Here, we show that the mRNA of latent TGF-β1 is expressed throughout amelogenesis. Latent TGF-β1 is activated by matrix metalloproteinase 20 (MMP20), coinciding wit...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025654/ https://www.ncbi.nlm.nih.gov/pubmed/27633089 http://dx.doi.org/10.1038/srep33644 |
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author | Kobayashi-Kinoshita, Saeko Yamakoshi, Yasuo Onuma, Kazuo Yamamoto, Ryuji Asada, Yoshinobu |
author_facet | Kobayashi-Kinoshita, Saeko Yamakoshi, Yasuo Onuma, Kazuo Yamamoto, Ryuji Asada, Yoshinobu |
author_sort | Kobayashi-Kinoshita, Saeko |
collection | PubMed |
description | Transforming growth factor-β1 (TGF-β1) is present in porcine enamel extracts and is critical for proper mineralization of tooth enamel. Here, we show that the mRNA of latent TGF-β1 is expressed throughout amelogenesis. Latent TGF-β1 is activated by matrix metalloproteinase 20 (MMP20), coinciding with amelogenin processing by the same proteinase. Activated TGF-β1 binds to the major amelogenin cleavage products, particularly the neutral-soluble P103 amelogenin, to maintain its activity. The P103 amelogenin-TGF-β1 complex binds to TGFBR1 to induce TGF-β1 signalling. The P103 amelogenin-TGF-β1 complex is slowly cleaved by kallikrein 4 (KLK4), which is secreted into the transition- and maturation-stage enamel matrix, thereby reducing TGF-β1 activity. To exert the multiple biological functions of TGF-β1 for amelogenesis, we propose that TGF-β1 is activated or inactivated by MMP20 or KLK4 and that the amelogenin cleavage product is necessary for the in-solution mobility of TGF-β1, which is necessary for binding to its receptor on ameloblasts and retention of its activity. |
format | Online Article Text |
id | pubmed-5025654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50256542016-09-22 TGF-β1 autocrine signalling and enamel matrix components Kobayashi-Kinoshita, Saeko Yamakoshi, Yasuo Onuma, Kazuo Yamamoto, Ryuji Asada, Yoshinobu Sci Rep Article Transforming growth factor-β1 (TGF-β1) is present in porcine enamel extracts and is critical for proper mineralization of tooth enamel. Here, we show that the mRNA of latent TGF-β1 is expressed throughout amelogenesis. Latent TGF-β1 is activated by matrix metalloproteinase 20 (MMP20), coinciding with amelogenin processing by the same proteinase. Activated TGF-β1 binds to the major amelogenin cleavage products, particularly the neutral-soluble P103 amelogenin, to maintain its activity. The P103 amelogenin-TGF-β1 complex binds to TGFBR1 to induce TGF-β1 signalling. The P103 amelogenin-TGF-β1 complex is slowly cleaved by kallikrein 4 (KLK4), which is secreted into the transition- and maturation-stage enamel matrix, thereby reducing TGF-β1 activity. To exert the multiple biological functions of TGF-β1 for amelogenesis, we propose that TGF-β1 is activated or inactivated by MMP20 or KLK4 and that the amelogenin cleavage product is necessary for the in-solution mobility of TGF-β1, which is necessary for binding to its receptor on ameloblasts and retention of its activity. Nature Publishing Group 2016-09-16 /pmc/articles/PMC5025654/ /pubmed/27633089 http://dx.doi.org/10.1038/srep33644 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kobayashi-Kinoshita, Saeko Yamakoshi, Yasuo Onuma, Kazuo Yamamoto, Ryuji Asada, Yoshinobu TGF-β1 autocrine signalling and enamel matrix components |
title | TGF-β1 autocrine signalling and enamel matrix components |
title_full | TGF-β1 autocrine signalling and enamel matrix components |
title_fullStr | TGF-β1 autocrine signalling and enamel matrix components |
title_full_unstemmed | TGF-β1 autocrine signalling and enamel matrix components |
title_short | TGF-β1 autocrine signalling and enamel matrix components |
title_sort | tgf-β1 autocrine signalling and enamel matrix components |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025654/ https://www.ncbi.nlm.nih.gov/pubmed/27633089 http://dx.doi.org/10.1038/srep33644 |
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