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A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization

A sorbitol dehydrogenase (GoSLDH) from Gluconobacter oxydans G624 (G. oxydans G624) was expressed in Escherichia coli BL21(DE3)-CodonPlus RIL. The complete 1455-bp codon-optimized gene was amplified, expressed, and thoroughly characterized for the first time. GoSLDH exhibited K(m) and k(cat) values...

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Detalles Bibliográficos
Autores principales: Kim, Tae-Su, Patel, Sanjay K. S., Selvaraj, Chandrabose, Jung, Woo-Suk, Pan, Cheol-Ho, Kang, Yun Chan, Lee, Jung-Kul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025769/
https://www.ncbi.nlm.nih.gov/pubmed/27633501
http://dx.doi.org/10.1038/srep33438