Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidig...

Descripción completa

Detalles Bibliográficos
Autores principales: Genshaft, Alex S, Li, Shuqiang, Gallant, Caroline J., Darmanis, Spyros, Prakadan, Sanjay M., Ziegler, Carly G. K., Lundberg, Martin, Fredriksson, Simon, Hong, Joyce, Regev, Aviv, Livak, Kenneth J., Landegren, Ulf, Shalek, Alex K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027636/
https://www.ncbi.nlm.nih.gov/pubmed/27640647
http://dx.doi.org/10.1186/s13059-016-1045-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027636/
https://www.ncbi.nlm.nih.gov/pubmed/27640647
http://dx.doi.org/10.1186/s13059-016-1045-6

Ejemplares similares