Evaluation of Lysis Methods for the Extraction of Bacterial DNA for Analysis of the Vaginal Microbiota

BACKGROUND: Recent studies on the vaginal microbiota have employed molecular techniques such as 16S rRNA gene sequencing to describe the bacterial community as a whole. These techniques require the lysis of bacterial cells to release DNA before purification and PCR amplification of the 16S rRNA gene...

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Detalles Bibliográficos
Autores principales: Gill, Christina, van de Wijgert, Janneke H. H. M., Blow, Frances, Darby, Alistair C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028042/
https://www.ncbi.nlm.nih.gov/pubmed/27643503
http://dx.doi.org/10.1371/journal.pone.0163148

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028042/
https://www.ncbi.nlm.nih.gov/pubmed/27643503
http://dx.doi.org/10.1371/journal.pone.0163148

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