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A simplified and robust protocol for immunoglobulin expression in E scherichia coli cell‐free protein synthesis systems
Cell‐free protein synthesis (CFPS) systems allow for robust protein expression with easy manipulation of conditions to improve protein yield and folding. Recent technological developments have significantly increased the productivity and reduced the operating costs of CFPS systems, such that they ca...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029582/ https://www.ncbi.nlm.nih.gov/pubmed/25826247 http://dx.doi.org/10.1002/btpr.2082 |