Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9

Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal...

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Detalles Bibliográficos
Autores principales: Iwata, Satoru, Yoshina, Sawako, Suehiro, Yuji, Hori, Sayaka, Mitani, Shohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030659/
https://www.ncbi.nlm.nih.gov/pubmed/27650892
http://dx.doi.org/10.1038/srep33840
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author Iwata, Satoru
Yoshina, Sawako
Suehiro, Yuji
Hori, Sayaka
Mitani, Shohei
author_facet Iwata, Satoru
Yoshina, Sawako
Suehiro, Yuji
Hori, Sayaka
Mitani, Shohei
author_sort Iwata, Satoru
collection PubMed
description Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes.
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spelling pubmed-50306592016-09-26 Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9 Iwata, Satoru Yoshina, Sawako Suehiro, Yuji Hori, Sayaka Mitani, Shohei Sci Rep Article Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes. Nature Publishing Group 2016-09-21 /pmc/articles/PMC5030659/ /pubmed/27650892 http://dx.doi.org/10.1038/srep33840 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Iwata, Satoru
Yoshina, Sawako
Suehiro, Yuji
Hori, Sayaka
Mitani, Shohei
Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9
title Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9
title_full Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9
title_fullStr Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9
title_full_unstemmed Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9
title_short Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9
title_sort engineering new balancer chromosomes in c. elegans via crispr/cas9
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030659/
https://www.ncbi.nlm.nih.gov/pubmed/27650892
http://dx.doi.org/10.1038/srep33840
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