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MSP-HTPrimer: a high-throughput primer design tool to improve assay design for DNA methylation analysis in epigenetics

BACKGROUND: Bisulfite (BS) conversion-based and methylation-sensitive restriction enzyme (MSRE)-based PCR methods have been the most commonly used techniques for locus-specific DNA methylation analysis. However, both methods have advantages and limitations. Thus, an integrated approach would be extr...

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Detalles Bibliográficos
Autores principales: Pandey, Ram Vinay, Pulverer, Walter, Kallmeyer, Rainer, Beikircher, Gabriel, Pabinger, Stephan, Kriegner, Albert, Weinhäusel, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031341/
https://www.ncbi.nlm.nih.gov/pubmed/27688817
http://dx.doi.org/10.1186/s13148-016-0269-3