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18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA

The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis. We compared (i) samples from...

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Detalles Bibliográficos
Autores principales: Leonard, Cory Ann, Meli, Marina L., Novacco, Marilisa, Borel, Nicole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031817/
https://www.ncbi.nlm.nih.gov/pubmed/27672657
http://dx.doi.org/10.1155/2016/6104323