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18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA
The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis. We compared (i) samples from...
Autores principales: | Leonard, Cory Ann, Meli, Marina L., Novacco, Marilisa, Borel, Nicole |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031817/ https://www.ncbi.nlm.nih.gov/pubmed/27672657 http://dx.doi.org/10.1155/2016/6104323 |
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