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Intein mediated hyper-production of authentic human basic fibroblast growth factor in Escherichia coli
Human basic fibroblast growth factor is a functionally versatile but very expensive polypeptide. In this communication, employing a novel amplification method for the target gene and genetic optimization of a previously engineered expression construct, pWK3R, together with a refined fed-batch fermen...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032022/ https://www.ncbi.nlm.nih.gov/pubmed/27653667 http://dx.doi.org/10.1038/srep33948 |