Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation

Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, a...

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Detalles Bibliográficos
Autores principales: Loch, Joanna I., Bonarek, Piotr, Tworzydło, Magdalena, Polit, Agnieszka, Hawro, Barbara, Łach, Aneta, Ludwin, Eryk, Lewiński, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035327/
https://www.ncbi.nlm.nih.gov/pubmed/27380951
http://dx.doi.org/10.1007/s12033-016-9960-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035327/
https://www.ncbi.nlm.nih.gov/pubmed/27380951
http://dx.doi.org/10.1007/s12033-016-9960-z

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