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Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation
Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, a...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035327/ https://www.ncbi.nlm.nih.gov/pubmed/27380951 http://dx.doi.org/10.1007/s12033-016-9960-z |