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A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq

BACKGROUND: The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision. RESULTS: We describe a method for sequencing near full-l...

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Detalles Bibliográficos
Autores principales: Burke, Catherine M., Darling, Aaron E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036073/
https://www.ncbi.nlm.nih.gov/pubmed/27688981
http://dx.doi.org/10.7717/peerj.2492