Validation of picogram- and femtogram-input DNA libraries for microscale metagenomics

High-throughput sequencing libraries are typically limited by the requirement for nanograms to micrograms of input DNA. This bottleneck impedes the microscale analysis of ecosystems and the exploration of low biomass samples. Current methods for amplifying environmental DNA to bypass this bottleneck...

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Detalles Bibliográficos
Autores principales: Rinke, Christian, Low, Serene, Woodcroft, Ben J., Raina, Jean-Baptiste, Skarshewski, Adam, Le, Xuyen H., Butler, Margaret K., Stocker, Roman, Seymour, Justin, Tyson, Gene W., Hugenholtz, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036114/
https://www.ncbi.nlm.nih.gov/pubmed/27688978
http://dx.doi.org/10.7717/peerj.2486

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036114/
https://www.ncbi.nlm.nih.gov/pubmed/27688978
http://dx.doi.org/10.7717/peerj.2486

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