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Stochastic emergence of multiple intermediates detected by single-molecule quasi-static mechanical unfolding of protein
Experimental probing of a protein-folding energy landscape can be challenging, and energy landscapes comprising multiple intermediates have not yet been defined. Here, we quasi-statically unfolded single molecules of staphylococcal nuclease by constant-rate mechanical stretching with a feedback posi...
Autores principales: | Fukagawa, Akihiro, Hiroshima, Michio, Sakane, Isao, Tokunaga, Makio |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society of Japan (BSJ)
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036639/ https://www.ncbi.nlm.nih.gov/pubmed/27857576 http://dx.doi.org/10.2142/biophysics.5.25 |
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