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Uracil-DNA glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination

Here, we describe a uracil-DNA glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previous...

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Detalles Bibliográficos
Autores principales: Kim, Eun-Mi, Jeon, Hyo-Sung, Kim, Ji-Jung, Shin, Yeun-Kyung, Lee, Youn-Jeong, Yeo, Sang-Geon, Park, Choi-Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037312/
https://www.ncbi.nlm.nih.gov/pubmed/26726027
http://dx.doi.org/10.4142/jvs.2016.17.3.421