Cargando…

Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar

Chickpea (Cicer arietinum) is a legume of the family Fabaceae, subfamily Faboideae. In Egypt, chickpea seeds are usually consumed at raw green and tender stage, or in the form of mature dry seeds. In our previous study, ‘Giza 1’ seeds exhibited stronger antioxidant activity and higher total phenol c...

Descripción completa

Detalles Bibliográficos
Autores principales: Mekky, Reham H., Fayed, Mostafa R., El-Gindi, Mohamed R., Abdel-Monem, Azza R., Contreras, María del Mar, Segura-Carretero, Antonio, Abdel-Sattar, Essam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040119/
https://www.ncbi.nlm.nih.gov/pubmed/27733831
http://dx.doi.org/10.3389/fphar.2016.00344
_version_ 1782456192380960768
author Mekky, Reham H.
Fayed, Mostafa R.
El-Gindi, Mohamed R.
Abdel-Monem, Azza R.
Contreras, María del Mar
Segura-Carretero, Antonio
Abdel-Sattar, Essam
author_facet Mekky, Reham H.
Fayed, Mostafa R.
El-Gindi, Mohamed R.
Abdel-Monem, Azza R.
Contreras, María del Mar
Segura-Carretero, Antonio
Abdel-Sattar, Essam
author_sort Mekky, Reham H.
collection PubMed
description Chickpea (Cicer arietinum) is a legume of the family Fabaceae, subfamily Faboideae. In Egypt, chickpea seeds are usually consumed at raw green and tender stage, or in the form of mature dry seeds. In our previous study, ‘Giza 1’ seeds exhibited stronger antioxidant activity and higher total phenol content than those from other Egyptian cultivars. In order to assess the biological potential of ‘Giza 1’ seeds in vivo, the extraction procedure was reproduced here. The extract was standardized using liquid chromatography coupled to diode array detector and tandem mass spectrometry (MS/MS) to evaluate their hepatoprotective effect on carbon tetrachloride (CCl(4))-induced hepatotoxicity in rats and acute toxicity. Administration of the extract to rats in doses up to 2 g/Kg) did not cause any mortalities or observable signs of toxicity. Further, the plant extract showed a strong hepatoprotective activity based on assessing serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and levels of albumen, globulin, total protein, total cholesterol, high density lipoprotein, triglycerides, and low density lipoprotein. The antioxidative activity was evaluated by assessing hepatic catalase and superoxide dismutase activity as well as reduced glutathione, and malondialdehyde levels. Additionally, anti-inflammatory activity was observed as the extract significantly lowered the hepatic tumor necrosis factor α content. Histopathological examination of liver tissues indicated that the extract-treated animals showed almost normal hepatic architecture with fewer pathological changes. In conclusion, the current results suggest that the chickpea extract possesses an excellent safety profile with very low acute toxicity. Also, it exhibits a significant hepatoprotective effect against CCl(4)-induced liver injury in rats. This can be attributed, at least partly, to the antioxidant and anti-inflammatory activity of the isoflavones and phenolic acids content of the extract.
format Online
Article
Text
id pubmed-5040119
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-50401192016-10-12 Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar Mekky, Reham H. Fayed, Mostafa R. El-Gindi, Mohamed R. Abdel-Monem, Azza R. Contreras, María del Mar Segura-Carretero, Antonio Abdel-Sattar, Essam Front Pharmacol Pharmacology Chickpea (Cicer arietinum) is a legume of the family Fabaceae, subfamily Faboideae. In Egypt, chickpea seeds are usually consumed at raw green and tender stage, or in the form of mature dry seeds. In our previous study, ‘Giza 1’ seeds exhibited stronger antioxidant activity and higher total phenol content than those from other Egyptian cultivars. In order to assess the biological potential of ‘Giza 1’ seeds in vivo, the extraction procedure was reproduced here. The extract was standardized using liquid chromatography coupled to diode array detector and tandem mass spectrometry (MS/MS) to evaluate their hepatoprotective effect on carbon tetrachloride (CCl(4))-induced hepatotoxicity in rats and acute toxicity. Administration of the extract to rats in doses up to 2 g/Kg) did not cause any mortalities or observable signs of toxicity. Further, the plant extract showed a strong hepatoprotective activity based on assessing serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and levels of albumen, globulin, total protein, total cholesterol, high density lipoprotein, triglycerides, and low density lipoprotein. The antioxidative activity was evaluated by assessing hepatic catalase and superoxide dismutase activity as well as reduced glutathione, and malondialdehyde levels. Additionally, anti-inflammatory activity was observed as the extract significantly lowered the hepatic tumor necrosis factor α content. Histopathological examination of liver tissues indicated that the extract-treated animals showed almost normal hepatic architecture with fewer pathological changes. In conclusion, the current results suggest that the chickpea extract possesses an excellent safety profile with very low acute toxicity. Also, it exhibits a significant hepatoprotective effect against CCl(4)-induced liver injury in rats. This can be attributed, at least partly, to the antioxidant and anti-inflammatory activity of the isoflavones and phenolic acids content of the extract. Frontiers Media S.A. 2016-09-28 /pmc/articles/PMC5040119/ /pubmed/27733831 http://dx.doi.org/10.3389/fphar.2016.00344 Text en Copyright © 2016 Mekky, Fayed, El-Gindi, Abdel-Monem, Contreras, Segura-Carretero and Abdel-Sattar. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Mekky, Reham H.
Fayed, Mostafa R.
El-Gindi, Mohamed R.
Abdel-Monem, Azza R.
Contreras, María del Mar
Segura-Carretero, Antonio
Abdel-Sattar, Essam
Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar
title Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar
title_full Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar
title_fullStr Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar
title_full_unstemmed Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar
title_short Hepatoprotective Effect and Chemical Assessment of a Selected Egyptian Chickpea Cultivar
title_sort hepatoprotective effect and chemical assessment of a selected egyptian chickpea cultivar
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040119/
https://www.ncbi.nlm.nih.gov/pubmed/27733831
http://dx.doi.org/10.3389/fphar.2016.00344
work_keys_str_mv AT mekkyrehamh hepatoprotectiveeffectandchemicalassessmentofaselectedegyptianchickpeacultivar
AT fayedmostafar hepatoprotectiveeffectandchemicalassessmentofaselectedegyptianchickpeacultivar
AT elgindimohamedr hepatoprotectiveeffectandchemicalassessmentofaselectedegyptianchickpeacultivar
AT abdelmonemazzar hepatoprotectiveeffectandchemicalassessmentofaselectedegyptianchickpeacultivar
AT contrerasmariadelmar hepatoprotectiveeffectandchemicalassessmentofaselectedegyptianchickpeacultivar
AT seguracarreteroantonio hepatoprotectiveeffectandchemicalassessmentofaselectedegyptianchickpeacultivar
AT abdelsattaressam hepatoprotectiveeffectandchemicalassessmentofaselectedegyptianchickpeacultivar