A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish

Contemporary improvements in the type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system offer a convenient way for genome editing in zebrafish. However, the low efficiencies of genome editing and germline transmission require a time-intensi...

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Detalles Bibliográficos
Autores principales: Xie, Shao-Lin, Bian, Wan-Ping, Wang, Chao, Junaid, Muhammad, Zou, Ji-Xing, Pei, De-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041118/
https://www.ncbi.nlm.nih.gov/pubmed/27680290
http://dx.doi.org/10.1038/srep34555

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041118/
https://www.ncbi.nlm.nih.gov/pubmed/27680290
http://dx.doi.org/10.1038/srep34555

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