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A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish
Contemporary improvements in the type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system offer a convenient way for genome editing in zebrafish. However, the low efficiencies of genome editing and germline transmission require a time-intensi...
Autores principales: | Xie, Shao-Lin, Bian, Wan-Ping, Wang, Chao, Junaid, Muhammad, Zou, Ji-Xing, Pei, De-Sheng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041118/ https://www.ncbi.nlm.nih.gov/pubmed/27680290 http://dx.doi.org/10.1038/srep34555 |
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