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Large-scale tissue clearing (PACT): Technical evaluation and new perspectives in immunofluorescence, histology, and ultrastructure

Novel techniques, like CLARITY and PACT, render large tissue specimens transparent and thereby suitable for microscopic analysis. We used these techniques to evaluate their potential in the intestine as an exemplary organ with a complex tissue composition. Immunohistochemistry, light sheet-, and con...

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Detalles Bibliográficos
Autores principales: Neckel, Peter H., Mattheus, Ulrich, Hirt, Bernhard, Just, Lothar, Mack, Andreas F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041186/
https://www.ncbi.nlm.nih.gov/pubmed/27680942
http://dx.doi.org/10.1038/srep34331