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Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System

CRISPR/Cas9 system has become a new versatile technology for genome engineering in various species. To achieve targeted modifications at the same site in both human and mice genomes by a CRISPR/Cas9 nuclease, we designed two target sites in conserved regions of vitamin D receptor (VDR) gene, which c...

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Detalles Bibliográficos
Autores principales:	Zhang, Tao, Yin, Yajun, Liu, Huan, Du, Weili, Ren, Chonghua, Wang, Ling, Lu, Hongzhao, Zhang, Zhiying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042489/ https://www.ncbi.nlm.nih.gov/pubmed/27685656 http://dx.doi.org/10.1371/journal.pone.0163551

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